RNA
Part:BBa_K4811019:Design
Designed by: Kasper Krunderup Jakobsen Group: iGEM23_DTU-Denmark (2023-10-03)
PT7-TMS(Kan1)-TT7
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 43
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 43
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 43
Illegal XhoI site found at 50
Illegal XhoI site found at 86 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 43
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 43
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
As of 2023, the TMS is a novel BioBrick, and not much is known about the specifics of TMS to RBS binding, as well as how an aptamer insertion into the D-loop will alter this binding, both in presence and absence of the ligand.
Source
The part was ordered as a gBlock from IDT.
The TMS was developed by Avishek Paul during their PhD thesis, see the publication "Modular and Versatile Trans-Encoded Genetic Switches", A. Paul, E. M. Warszawik, M. Loznik, A. J. Boersma, A. Herrmann, Angew. Chem. Int. Ed. 2020, 59, 20328.