Part:BBa_K4811018:Design
PT7-TMS(GFP1)-TT7
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 33
Illegal BglII site found at 122
Illegal XhoI site found at 131
Illegal XhoI site found at 167 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
As of 2023, the TMS is a novel BioBrick, and not much is known about the specifics of TMS to RBS binding, as well as how an aptamer insertion into the D-loop will alter this binding, both in presence and absence of the ligand.
Source
The part was ordered as a gBlock from IDT,
The TMS was developed by Avishek Paul during their PhD thesis, see the publication "Modular and Versatile Trans-Encoded Genetic Switches", A. Paul, E. M. Warszawik, M. Loznik, A. J. Boersma, A. Herrmann, Angew. Chem. Int. Ed. 2020, 59, 20328.
For information on the GFP-sensitive aptamer, see the publication "RNA aptamers that functionally interact with green fluorescent protein and its derivatives", B. Shui, A. Ozer, W. Zipfel, N. Sahu, A. Singh, J. T. Lis, H. Shi, M. I. Kotlikoff, Nucleic Acids Res. 2012, 40, e39