RNA

Part:BBa_K4811011:Design

Designed by: Kasper Krunderup Jakobsen   Group: iGEM23_DTU-Denmark   (2023-09-25)


TMS(Theo2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 87
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The RNA has to fold as a tRNA would


Source

The TMS was developed by Avishek Paul during their PHD thesis, see the publication "Modular and Versatile Trans-Encoded Genetic Switches", A. Paul, E. M. Warszawik, M. Loznik, A. J. Boersma, A. Herrmann, Angew. Chem. Int. Ed. 2020, 59, 20328.

The Theophylline sensitive aptamer was adapted from Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz, Wolfgang Hillen, A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo, Nucleic Acids Research, Volume 32, Issue 4, 15 February 2004, Pages 1610–1614, https://doi.org/10.1093/nar/gkh321

References