RNA

Part:BBa_K4811002:Design

Designed by: Kasper Krunderup Jakobsen   Group: iGEM23_DTU-Denmark   (2023-09-04)


TMS: tRNA Mimicking Structure


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 77
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence has to fold as a tRNA. The D-loop can be exchanged to an aptamer to aquire additional functionality, where there is evidence for a competition between aptamer-ligand binding, and TMS-RBS binding.


Source

The part was developed by Avishek Paul during their PHD thesis, see the publication Modular and Versatile Trans-Encoded Genetic Switches, A. Paul, E. M. Warszawik, M. Loznik, A. J. Boersma, A. Herrmann, Angew. Chem. Int. Ed. 2020, 59, 20328.

References