![](https://parts.igem.org/images/partbypart/icon_primer.png)
Primer
Part:BBa_K4810025:Design
Designed by: Theodoros Desiniotis Group: iGEM23_Athens (2023-10-11)
PCR reverse primer for modification of VOC-binding pVIII M13 protein
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 7
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 7
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 7
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 7
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Primer contains PstI restriction site used to ligate digested M13KE vector to re-circularize
Source
Synthetic DNA