Primer

Part:BBa_K4810025

Designed by: Theodoros Desiniotis   Group: iGEM23_Athens   (2023-10-11)


PCR reverse primer for modification of VOC-binding pVIII M13 protein

Reverse primer used to induce substitution mutations near the N-terminus of M13 pVIII protein in order to modify the N-terminal peptide sequence used to create VOC-binding pVIII protein

Part "BBa_K4810025" used as reverse primer for parts: "BBa_K4810020", "BBa_K4810021", "BBa_K4810022", "BBa_K4810023" and "BBa_K4810024"

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 7
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 7
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 7
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 7
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None