Primer

Part:BBa_K4810023

Designed by: Theodoros Desiniotis   Group: iGEM23_Athens   (2023-10-11)


PCR primer for modification of pVIII M13 protein to detect Octadecanal

PCR forward primer designed to modify M13 pVIII protein near the N-terminus. Modification replaces EGDD with DMWVK (sequence derived through Molecular Docking using Autodock Vina) for the purpose of creating a Octadecanal-binding pVIII, implemented as a biosensor when M13 phages self-assemble on a gold-coated substrate.

Part "BBa_K4810025" used as reverse primer


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 7
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 7
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 7
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 7
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None