![](https://parts.igem.org/images/partbypart/icon_plasmid.png)
Plasmid
Part:BBa_K4810010:Design
Designed by: Theodoros Desiniotis Group: iGEM23_Athens (2023-10-11)
M13KE vector modified for production of M13 Phages detecting “Hippuric Acid”
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 5293
Illegal BamHI site found at 6670 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3998
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The original sequence of pVIII protein consists of 222bp coding for 73 amino acids. First 23 amino acids are cleaved by a signal-peptidase inside E.coli cells. Thus in order to introduce the modified hexapeptide (or any peptide) in the N-terminal of the functional protein, the appropriate codons should be introduced from the codon #24 and onwards in the sequence of the pVIII protein.
Source
M13KE DNA (unmodified plasmid)