![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4810001:Design
Designed by: Theodoros Desiniotis, Ioannis Stamelos, Aikaterini Spanou Group: iGEM23_Athens (2023-10-10)
Modified M13 pVIII protein for Hippuric Acid binding
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Original sequence consists of 222bp coding for 73 amino acids. First 23 amino acids are cleaved by a signal-peptidase inside E.coli cells. Thus in order to introduce the modified hexapeptide (or any peptide) in the N-terminal of the functional protein, the appropriate codons should be introduced from the codon #24 and onwards in the sequence of the pVIII protein.
Source
unmodified sequence from M13KE vector