Composite

Part:BBa_K4807027:Design

Designed by: Vaios Styliaras, Efi Chatzopoulou   Group: iGEM23_IOANNINA   (2023-10-11)


Rluc8 insert


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 667


Design Notes

Minor base modifications have been made for proper Primer Design, for keeping translational frame intact etc


Source

Renilla Reniformis for Rluc8 Gene, E.coli for regulatory elements.

References