Part:BBa_K4806214:Design

POR/CYP3A4 tandem with HA-tag for Chlamydomonas reinhardtii (Phytobrick)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 3722
  Illegal PstI site found at 4982
  Illegal PstI site found at 5042
  Illegal PstI site found at 5701
  Illegal PstI site found at 8870
  Illegal PstI site found at 9192
  Illegal PstI site found at 9252
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 2618
  Illegal NheI site found at 4907
  Illegal NheI site found at 7273
  Illegal PstI site found at 3722
  Illegal PstI site found at 4982
  Illegal PstI site found at 5042
  Illegal PstI site found at 5701
  Illegal PstI site found at 8870
  Illegal PstI site found at 9192
  Illegal PstI site found at 9252
  Illegal NotI site found at 5347
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 2899
  Illegal XhoI site found at 7554
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 3722
  Illegal PstI site found at 4982
  Illegal PstI site found at 5042
  Illegal PstI site found at 5701
  Illegal PstI site found at 8870
  Illegal PstI site found at 9192
  Illegal PstI site found at 9252
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 3722
  Illegal PstI site found at 4982
  Illegal PstI site found at 5042
  Illegal PstI site found at 5701
  Illegal PstI site found at 8870
  Illegal PstI site found at 9192
  Illegal PstI site found at 9252
  Illegal NgoMIV site found at 1270
  Illegal NgoMIV site found at 1452
  Illegal NgoMIV site found at 6630
  Illegal NgoMIV site found at 9114
  Illegal NgoMIV site found at 10618
  Illegal AgeI site found at 2637
  Illegal AgeI site found at 7292
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part has been codon optimized for Chlamydomonas reinhardtii and it is compatible with Phytobricks/MoClo parts.

Source

This composite part is a combination of the following parts:

• 1. AβSAP(i)-promotor (A1-B2) (BBa_K4806013)
• 2.1 CYP3A4 gene (B3-B4) (BBa_K4806000)
• 2.2 POR gene (B3-B4) (BBa_K4806003)
• 3. HA-tag (B5) (BBa_K3002017)^*
• 4. tRPL23-terminator (B6-C1) (BBa_K3002006)^*

Additionally, connectors were used to create the MoClo overhangs:

• 1. Connector B2-B3 (BBa_K3002303)^*
• 2. Connector B4-B5 (BBa_K3002304)^*
• 3. Connector B5-B6 (BBa_K3002305)^*
• 4. Connector F1-F2 (BBa_K4806018)
• 5. Connector F2-F3 (BBa_K4806019)
• 6. Connector F3-F4 (BBa_K4806020)
• 7. Endlinker (BBa_K4806016)

The ^* marked parts were not created by us. Our results can be found on the experience page of each part.