Composite

Part:BBa_K4806214:Design

Designed by: Luca Langenberg   Group: iGEM23_RPTU-Kaiserslautern   (2023-10-04)


POR/CYP3A4 tandem with HA-tag for Chlamydomonas reinhardtii (Phytobrick)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3722
    Illegal PstI site found at 4982
    Illegal PstI site found at 5042
    Illegal PstI site found at 5701
    Illegal PstI site found at 8870
    Illegal PstI site found at 9192
    Illegal PstI site found at 9252
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2618
    Illegal NheI site found at 4907
    Illegal NheI site found at 7273
    Illegal PstI site found at 3722
    Illegal PstI site found at 4982
    Illegal PstI site found at 5042
    Illegal PstI site found at 5701
    Illegal PstI site found at 8870
    Illegal PstI site found at 9192
    Illegal PstI site found at 9252
    Illegal NotI site found at 5347
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2899
    Illegal XhoI site found at 7554
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3722
    Illegal PstI site found at 4982
    Illegal PstI site found at 5042
    Illegal PstI site found at 5701
    Illegal PstI site found at 8870
    Illegal PstI site found at 9192
    Illegal PstI site found at 9252
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3722
    Illegal PstI site found at 4982
    Illegal PstI site found at 5042
    Illegal PstI site found at 5701
    Illegal PstI site found at 8870
    Illegal PstI site found at 9192
    Illegal PstI site found at 9252
    Illegal NgoMIV site found at 1270
    Illegal NgoMIV site found at 1452
    Illegal NgoMIV site found at 6630
    Illegal NgoMIV site found at 9114
    Illegal NgoMIV site found at 10618
    Illegal AgeI site found at 2637
    Illegal AgeI site found at 7292
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has been codon optimized for Chlamydomonas reinhardtii and it is compatible with Phytobricks/MoClo parts.

Source

This composite part is a combination of the following parts:

Additionally, connectors were used to create the MoClo overhangs:

The * marked parts were not created by us. Our results can be found on the experience page of each part.

References