Part:BBa_K4806206:Design

CYP2D6 gene with FLAG-tag for Chlamydomonas reinhardtii (Phytobrick)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1098
  Illegal PstI site found at 2454
  Illegal PstI site found at 2887
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 249
  Illegal PstI site found at 1098
  Illegal PstI site found at 2454
  Illegal PstI site found at 2887
  Illegal NotI site found at 1604
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 530
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1098
  Illegal PstI site found at 2454
  Illegal PstI site found at 2887
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1098
  Illegal PstI site found at 2454
  Illegal PstI site found at 2887
  Illegal NgoMIV site found at 1741
  Illegal NgoMIV site found at 2090
  Illegal NgoMIV site found at 3004
  Illegal NgoMIV site found at 3010
  Illegal NgoMIV site found at 3422
  Illegal AgeI site found at 268
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part has been codon optimized for Chlamydomonas reinhardtii and it is compatible with Phytobricks/MoClo parts.

Source

This composite part is a combination of the following parts:

• 1. AβSAP(i)-promotor (A1-B2) (BBa_K4806013)
• 2. CYP2D6 gene (B3-B4) (BBa_K4806001)
• 3. FLAG-tag (B5) (BBa_K4806012)
• 4. tRPL23-terminator (B6-C1) (BBa_K3002006)^*

Additionally, connectors were used to create the MoClo overhangs:

• 1. Connector B2-B3 (BBa_K3002303)^*
• 2. Connector B4-B5 (BBa_K3002304)^*
• 3. Connector B5-B6 (BBa_K3002305)^*

The ^* marked parts were not created by us. Our results can be found on the experience page of each part.