Composite

Part:BBa_K4806108:Design

Designed by: Luca Langenberg   Group: iGEM23_RPTU-Kaiserslautern   (2023-10-02)


CYPCamC gene with HA-tag for Chlamydomonas reinhardtii (Phytobrick)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1023
    Illegal PstI site found at 1295
    Illegal PstI site found at 2019
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal PstI site found at 1023
    Illegal PstI site found at 1295
    Illegal PstI site found at 2019
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 530
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1023
    Illegal PstI site found at 1295
    Illegal PstI site found at 2019
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1023
    Illegal PstI site found at 1295
    Illegal PstI site found at 2019
    Illegal NgoMIV site found at 3040
    Illegal AgeI site found at 268
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has been codon optimized for Chlamydomonas reinhardtii and it is compatible with Phytobricks/MoClo parts.

Source

This composite part is a combination of the following parts:

Additionally, connectors were used to create the MoClo overhangs:

The * marked parts were not created by us. Our results can be found on the experience page of each part.

References