Part:BBa_K4806100:Design

Hygromycin Resistance for Chlamydomonas reinhardtii (Phytobrick)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1270
  Illegal NgoMIV site found at 1452
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part has been codon optimized for Chlamydomonas reinhardtii and it is compatible with Phytobricks/MoClo parts.

Source

This composite part is a combination of the following parts:

• 1. PSAD-promotor (A1-B2) (BBa_K3002001)^*
• 2. Hygromycin resistance gene (B3-B5) (BBa_K4806015)
• 3. PSAD-terminator (B6-C1) (BBa_K3002002)^*

Additionally, connectors were used to create the MoClo overhangs:

• 1. Connector B2-B3 (BBa_K3002303)^*
• 2. Connector B5-B6 (BBa_K3002305)^*

The ^* marked parts were not created by us. Our results can be found on the experience page of each part.