Coding

Part:BBa_K4806015:Design

Designed by: Luca Langenberg   Group: iGEM23_RPTU-Kaiserslautern   (2023-09-21)


Hygromycin resistance for Chlamydomonas reinhardtii (Phytobrick)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 455
    Illegal NgoMIV site found at 637
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon optimized for Chlamydomonas reinhardtii and compatible with Phytobricks/MoClo parts.

Source

Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. A modular cloning system for standardized assembly of multigene constructs. PLoS One. 2011 Feb 18;6(2):e16765. doi: 10.1371/journal.pone.0016765. PMID: 21364738; PMCID: PMC3041749.

References