Part:BBa_K4806011:Design
mtTP70C-transit peptide for Chlamydomonas reinhardtii (Phytobrick)
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 2
Illegal PstI site found at 183 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 2
Illegal PstI site found at 183 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 198
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 2
Illegal PstI site found at 183 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 2
Illegal PstI site found at 183 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is codon optimized for Chlamydomonas reinhardtii and compatible with Phytobricks/MoClo parts.
Source
The sequence was provided by the MoClo toolkit for Chlamydomonas reinhardtii.
Brick name: MTP CrHSP70C(i) (N-ter, CDS) (pCM0-057)
Source: Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.