Coding

Part:BBa_K4804000:Design

Designed by: Jing Shi   Group: iGEM23_Bioplus-China   (2023-08-22)


PETase-R


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 304
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 871
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 923
    Illegal NgoMIV site found at 965
    Illegal AgeI site found at 627
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PETase without stop codon


Source

PETase from Ideonella sakaiensis. PySp1 from Araneida

References