Composite

Part:BBa_K4803111:Design

Designed by: Kotaro MURAI   Group: iGEM23_UTokyo   (2023-10-10)


CD4signal-eGFP-FCS-CD28TMD-PPVcs-KKYL


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We performed codon optimization to ensure that this part is effectively expressed in human cells. Major restriction enzyme recognition sequences (EcoRI XbaI SpeI PstI NotI BsaⅠ SapⅠ) are removed from this part.

Source

This biobrick is created by combining Part:BBa_K4803005(CD4 signal), Part:BBa_K2559005(eGFP), Part:BBa_K4803012(Furin cleavage site), Part:BBa_K4803006(CD28 Transmembrane domain), Part:BBa_K4803013(PPV cleavage site), Part:BBa_K4803014(ER retention signal).