Composite

Part:BBa_K4803101:Design

Designed by: Kotaro MURAI   Group: iGEM23_UTokyo   (2023-10-10)


CD4signal-gp130ex-CD28TMD-TEVp


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1159
    Illegal SapI.rc site found at 1507


Design Notes

Major restriction enzyme recognition sequences (EcoRI XbaI SpeI PstI NotI BsaⅠ) are removed from this part.

Source

This biobrick is MESA Part created by combining Part:BBa_K4803005(CD4 signal), Part:BBa_K4803002(gp130 extracellular domain), Part:BBa_K4803006(CD28 Transmembrane domain), Part:BBa_K2969000(TEV protease).