Protein_Domain

Part:BBa_K4803003:Design

Designed by: Kotaro MURAI   Group: iGEM23_UTokyo   (2023-10-10)


FRB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 219
    Illegal XhoI site found at 4
    Illegal XhoI site found at 85
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We performed codon optimization to ensure effective expression of this part in human cells. We also used simulations to predict and confirm that the steric structure is not disrupted even when the amino acid sequence is only in the extracellular domain.

Major restriction enzyme recognition sequences (EcoRI XbaI SpeI PstI NotI BsaⅠ SapⅠ) are removed from this part.

Source

The sequence was obtained by codon optimization (IDT codon optimization tool) for FRB described in [1].

Reference

[1] Rivera, V. M., Wang, X., Wardwell, S., Courage, N. L., Volchuk, A., Keenan, T., ... & Clackson, T. (2000). Regulation of protein secretion through controlled aggregation in the endoplasmic reticulum. Science, 287(5454), 826-830. https://doi.org/10.1038/s41467-022-28971-9