Composite

Part:BBa_K4800053:Design

Designed by: Chenxi Ma   Group: iGEM23_NJTech-China-B   (2023-10-09)


pACYCDuet-sfp-MmCAR-YahK


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4801
    Illegal XbaI site found at 2084
    Illegal PstI site found at 5236
    Illegal PstI site found at 5263
    Illegal PstI site found at 6532
    Illegal PstI site found at 6559
    Illegal PstI site found at 6952
    Illegal PstI site found at 7448
    Illegal PstI site found at 8092
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4801
    Illegal NheI site found at 1243
    Illegal NheI site found at 8360
    Illegal PstI site found at 5236
    Illegal PstI site found at 5263
    Illegal PstI site found at 6532
    Illegal PstI site found at 6559
    Illegal PstI site found at 6952
    Illegal PstI site found at 7448
    Illegal PstI site found at 8092
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4801
    Illegal BglII site found at 5790
    Illegal BglII site found at 6416
    Illegal BglII site found at 6692
    Illegal BglII site found at 7383
    Illegal BamHI site found at 4773
    Illegal XhoI site found at 8332
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4801
    Illegal XbaI site found at 2084
    Illegal PstI site found at 5236
    Illegal PstI site found at 5263
    Illegal PstI site found at 6532
    Illegal PstI site found at 6559
    Illegal PstI site found at 6952
    Illegal PstI site found at 7448
    Illegal PstI site found at 8092
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4801
    Illegal XbaI site found at 2084
    Illegal PstI site found at 5236
    Illegal PstI site found at 5263
    Illegal PstI site found at 6532
    Illegal PstI site found at 6559
    Illegal PstI site found at 6952
    Illegal PstI site found at 7448
    Illegal PstI site found at 8092
    Illegal NgoMIV site found at 7060
    Illegal NgoMIV site found at 7122
    Illegal NgoMIV site found at 7339
    Illegal NgoMIV site found at 7786
    Illegal AgeI site found at 57
    Illegal AgeI site found at 1329
    Illegal AgeI site found at 1652
    Illegal AgeI site found at 4966
    Illegal AgeI site found at 5977
    Illegal AgeI site found at 6274
    Illegal AgeI site found at 7159
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Any restriction sites for EcoRI, XbaI, PstI, SpeI, and NotI have been removed.


Source

None

References