Composite
Part:BBa_K4800052
Designed by: Qiming Zou Group: iGEM23_NJTech-China-B (2023-10-08)
pTrc99a-YahK-QLH
To compare the activity of different transaminases, the DNA fragments of Ptrc-RBS-YahK were amplified and ligated into the plasmids of pTrc99a-QLH.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3624
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4351
Illegal NgoMIV site found at 4900
Illegal AgeI site found at 4380 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5283
Illegal BsaI site found at 6351
Illegal SapI.rc site found at 767
[edit]
Categories
Parameters
None |