Composite

Part:BBa_K4800049:Design

Designed by: Chenxi Ma   Group: iGEM23_NJTech-China-B   (2023-10-08)


pTrc99a-YahK-Spo3471


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3808
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3808
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3624
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3808
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3808
    Illegal NgoMIV site found at 4468
    Illegal AgeI site found at 4455
    Illegal AgeI site found at 4579
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5346
    Illegal BsaI site found at 6414
    Illegal SapI.rc site found at 767


Design Notes

Any restriction sites for EcoRI, XbaI, PstI, SpeI, and NotI have been removed.


Source

None

References