![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4800049:Design
Designed by: Chenxi Ma Group: iGEM23_NJTech-China-B (2023-10-08)
pTrc99a-YahK-Spo3471
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 3808
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 3808
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3624
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 3808
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 3808
Illegal NgoMIV site found at 4468
Illegal AgeI site found at 4455
Illegal AgeI site found at 4579 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5346
Illegal BsaI site found at 6414
Illegal SapI.rc site found at 767
Design Notes
Any restriction sites for EcoRI, XbaI, PstI, SpeI, and NotI have been removed.
Source
None