Composite
Part:BBa_K4800045:Design
Designed by: Chenxi Ma Group: iGEM23_NJTech-China-B (2023-10-08)
pTrc99a-SpuC
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 4051
Illegal PstI site found at 3704
Illegal PstI site found at 4063 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 3704
Illegal PstI site found at 4063 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4045
Illegal XhoI site found at 3998 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 4051
Illegal PstI site found at 3704
Illegal PstI site found at 4063 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 4051
Illegal PstI site found at 3704
Illegal PstI site found at 4063
Illegal AgeI site found at 2987
Illegal AgeI site found at 3440 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4247
Illegal BsaI site found at 5315
Illegal SapI.rc site found at 767
Design Notes
Any restriction sites for EcoRI, XbaI, PstI, SpeI, and NotI have been removed.
Source
None