Composite

Part:BBa_K4800044:Design

Designed by: Chenxi Ma   Group: iGEM23_NJTech-China-B   (2023-10-08)


pTrc99a-QLH


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3988
    Illegal PstI site found at 4000
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 4000
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3982
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3988
    Illegal PstI site found at 4000
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3988
    Illegal PstI site found at 4000
    Illegal NgoMIV site found at 3209
    Illegal NgoMIV site found at 3758
    Illegal AgeI site found at 3238
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4184
    Illegal BsaI site found at 5252
    Illegal SapI.rc site found at 767


Design Notes

Any restriction sites for EcoRI, XbaI, PstI, SpeI, and NotI have been removed.


Source

None

References