Composite
Part:BBa_K4800044:Design
Designed by: Chenxi Ma Group: iGEM23_NJTech-China-B (2023-10-08)
pTrc99a-QLH
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 3988
Illegal PstI site found at 4000 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 4000
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3982
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 3988
Illegal PstI site found at 4000 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 3988
Illegal PstI site found at 4000
Illegal NgoMIV site found at 3209
Illegal NgoMIV site found at 3758
Illegal AgeI site found at 3238 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4184
Illegal BsaI site found at 5252
Illegal SapI.rc site found at 767
Design Notes
Any restriction sites for EcoRI, XbaI, PstI, SpeI, and NotI have been removed.
Source
None