Composite

Part:BBa_K4800041:Design

Designed by: Chenxi Ma   Group: iGEM23_NJTech-China-B   (2023-10-08)


pTrc99a-PatA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4036
    Illegal PstI site found at 4048
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 4048
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4030
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4036
    Illegal PstI site found at 4048
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4036
    Illegal PstI site found at 4048
    Illegal NgoMIV site found at 2804
    Illegal AgeI site found at 3311
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4232
    Illegal BsaI site found at 5300
    Illegal SapI.rc site found at 767


Design Notes

Any restriction sites for EcoRI, XbaI, PstI, SpeI, and NotI have been removed.


Source

None

References