Composite

Part:BBa_K4800039:Design

Designed by: Chenxi Ma   Group: iGEM23_NJTech-China-B   (2023-10-08)


pTrc99a-Adhp


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3602
    Illegal PstI site found at 2582
    Illegal PstI site found at 3614
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2582
    Illegal PstI site found at 3614
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3596
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3602
    Illegal PstI site found at 2582
    Illegal PstI site found at 3614
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3602
    Illegal PstI site found at 2582
    Illegal PstI site found at 3614
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3798
    Illegal BsaI site found at 4866
    Illegal SapI.rc site found at 767


Design Notes

Any restriction sites for EcoRI, XbaI, PstI, SpeI, and NotI have been removed.


Source

None

References