Composite
Part:BBa_K4800038:Design
Designed by: Chenxi Ma Group: iGEM23_NJTech-China-B (2023-10-08)
pTrc99a-Hsd36
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 3353
Illegal PstI site found at 3365 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 3365
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3347
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 3353
Illegal PstI site found at 3365 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 3353
Illegal PstI site found at 3365
Illegal AgeI site found at 2611
Illegal AgeI site found at 2683
Illegal AgeI site found at 3292 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3549
Illegal BsaI site found at 4617
Illegal SapI.rc site found at 767
Design Notes
Any restriction sites for EcoRI, XbaI, PstI, SpeI, and NotI have been removed.
Source
None