Coding

Part:BBa_K4800023:Design

Designed by: Yao Yao   Group: iGEM23_NJTech-China-B   (2023-10-07)


MAB4714


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 454
    Illegal NgoMIV site found at 756
    Illegal NgoMIV site found at 946
    Illegal NgoMIV site found at 1309
    Illegal NgoMIV site found at 2364
    Illegal NgoMIV site found at 2743
    Illegal NgoMIV site found at 2968
    Illegal AgeI site found at 274
    Illegal AgeI site found at 493
    Illegal AgeI site found at 1516
    Illegal AgeI site found at 2236
    Illegal AgeI site found at 2790
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Any restriction sites for EcoRI, XbaI, PstI, SpeI, and NotI have been removed.


Source

MAB4714 is from Mycobacteroides abscessus.

References