Coding

Part:BBa_K4800022:Design

Designed by: Yao Yao   Group: iGEM23_NJTech-China-B   (2023-10-07)


MSMEG5739


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1107
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 225
    Illegal NgoMIV site found at 820
    Illegal NgoMIV site found at 828
    Illegal NgoMIV site found at 1240
    Illegal NgoMIV site found at 3340
    Illegal AgeI site found at 967
    Illegal AgeI site found at 1285
    Illegal AgeI site found at 2523
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Any restriction sites for EcoRI, XbaI, PstI, SpeI, and NotI have been removed.


Source

MSMEG5739 is from Mycolicibacterium smegmatis MC2 155.

References