DNA

Part:BBa_K4800010:Design

Designed by: Wenbin Zhao   Group: iGEM23_NJTech-China-B   (2023-10-06)


ackA-pta Donor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 969
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 969
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 969
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 969
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Any restriction sites for EcoRI, XbaI, PstI, SpeI, and NotI have been removed.



Source

ackA-pta Donor is from Escherichia coli.

References