Reporter

Part:BBa_K4799609:Design

Designed by: Wanjun Chen   Group: iGEM23_BGI-MammothEdu-China   (2023-10-10)


pAtMT1a_GUS_T-Nos


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 25
    Illegal SpeI site found at 1374
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 25
    Illegal NheI site found at 3183
    Illegal SpeI site found at 1374
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 25
    Illegal BglII site found at 1165
    Illegal BamHI site found at 1148
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 25
    Illegal SpeI site found at 1374
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 25
    Illegal SpeI site found at 1374
    Illegal NgoMIV site found at 1598
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1398
    Illegal BsaI.rc site found at 2013
    Illegal SapI.rc site found at 503
    Illegal SapI.rc site found at 2184


Design Notes

Test if the promoter will induce GUS express after cadmium treatment.



Source

plasmid

References