Composite

Part:BBa_K4790087:Design

Designed by: Hongyan Jing   Group: iGEM23_NNU-CHINA   (2023-10-11)


ASR7(node 115)/pET-22b(+)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 442
    Illegal PstI site found at 453
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 966
    Illegal PstI site found at 442
    Illegal PstI site found at 453
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 442
    Illegal PstI site found at 453
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 442
    Illegal PstI site found at 453
    Illegal NgoMIV site found at 143
    Illegal NgoMIV site found at 425
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

The original sequence of pET-22b was downloaded from NCBI. Node 115 was constructed through Ancestral Sequence Reconstruction (ASR).

References