Composite

Part:BBa_K4789006:Design

Designed by: Fangyuan Duan   Group: iGEM23_YiYe-China   (2023-09-23)


pcDNA-pre-miR-22


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 952
    Illegal XbaI site found at 991
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2313
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 952
    Illegal NheI site found at 895
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2313
    Illegal NotI site found at 978
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 952
    Illegal BglII site found at 12
    Illegal BamHI site found at 929
    Illegal XhoI site found at 985
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 952
    Illegal XbaI site found at 991
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2313
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 952
    Illegal XbaI site found at 991
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2313
    Illegal NgoMIV site found at 1423
    Illegal NgoMIV site found at 2764
    Illegal NgoMIV site found at 3047
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 882
    Illegal BsaI.rc site found at 4572
    Illegal SapI site found at 3492
    Illegal SapI.rc site found at 2613
    Illegal SapI.rc site found at 2823


Design Notes

pcDNA-pre-miR-22 was applied to overexpress miR-22 in cells.Its basic backbone is pcDNA3.0, and pre-miR-22 sequence was inserted into the basic plasmid.

Source

1

References

[1]Nejati Kazem,Alivand MohammadReza,Arabzadeh AmirAhmad,MicroRNA-22 in female malignancies: Focusing on breast, cervical, and ovarian cancers.[J] .Pathol Res Pract, 2021, 223: 153452.

[2]Konishi Hiromi,Hayashi Masami,Taniguchi Kohei et al. The therapeutic potential of exosomal miR-22 for cervical cancer radiotherapy.[J] .Cancer Biol Ther, 2020, 21: 1128-1135.

[3]Zhang Lu,Chen Bin,Ding Ding,Decreased microRNA-22 is associated with poor prognosis in cervical cancer.[J] .Int J Clin Exp Pathol, 2017, 10: 9515-9520.

[4] Nakamura Mayumi,Hayashi Masami,Konishi Hiromi et al. MicroRNA-22 enhances radiosensitivity in cervical cancer cell lines via direct inhibition of c-Myc binding protein, and the subsequent reduction in hTERT expression.[J] .Oncol Lett, 2020, 19: 2213-2222.

[5] Han Xiting,Wang Qian,Wang Yan et al. Long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1/microRNA-202-3p/periostin axis modulates invasion and epithelial-mesenchymal transition in human cervical cancer.[J] .J Cell Physiol, 2019, 234: 14170-14180.

[6] http://www.mircode.org/