Composite

Part:BBa_K4789005:Design

Designed by: Fangyuan Duan   Group: iGEM23_YiYe-China   (2023-09-23)


miR-145-sponge-pepper


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use the “Pepper ” to visualize LncRNA MALAT1 in order to reflect miRNA expression in vivo. The pepper-MALAT1 plasmid will emit green fluorescence when HBC is added. In the healthy people, there were fewer miR-145. So fewer miRNA could bind to MALAT1.More free MALAT1 will emit fluorescence through reporter “Pepper assay”.

Source

The plasmid of pCDNA3-MCS-nPepper was obtained from Naying Biotechnology Company.

References

[1] Chen Xianjun,Zhang Dasheng,Su Ni et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs.[J] .Nat Biotechnol, 2019, 37: 1287-1293.