Part:BBa_K4788004

FadO operator

FadO is an operator that can bind to the FadR protein. When there is a FadBA promoter in front of it, this binding inhibits the expression of the FadBA promoter. And oleoyl CoA metabolized from oleic acid can relieve this inhibition. The number and position of different FadO operators will change the threshold for de suppression.

Usage and Biology

It is a basic operator component that can combine with the FadR gene and FadBA promoter gene to form oleic acid inducers, exerting rich functions. And changing its position and quantity can change the induction threshold of the oleic acid inducer.

After forming composite components

The FadR protein expressed by the FadR gene can bind to the operator FadO following the FadBA promoter, which inhibits the initiation of FadBA. However, oleoyl CoA metabolized from oleic acid can relieve this inhibition and promote the expression of FadBA promoter. This allows it to be induced by oleic acid for expression. In practical applications, we can change the number and position of FadO operators to change their response threshold to oleic acid to achieve more precise regulatory effects.

Reference

Ma Y, Zheng X, Lin Y, Zhang L, Yuan Y, Wang H, Winterburn J, Wu F, Wu Q, Ye JW, Chen GQ. Engineering an oleic acid-induced system for Halomonas, E. coli and Pseudomonas. Metab Eng. 2022 Jul;72:325-336. doi: 10.1016/j.ymben.2022.04.003. Epub 2022 May 2. PMID: 35513297.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]