Composite

Part:BBa_K4786001:Design

Designed by: Yucheng Zhang   Group: iGEM23_Tongji-China   (2023-10-06)


trc promoter_SpdCas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1210
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3489
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed dCas9 induced by the trc promoter


Source

dCas9 sequence is from Cloning vector CRISPRi pRG_dCas9, complete sequence, through Genbank https://www.ncbi.nlm.nih.gov/nucleotide/MK253667.1?report=genbank&log$=nuclalign&blast_rank=1&RID=HZC0VV5D016


References