DNA

Part:BBa_K4781011

Designed by: Avijit Majumder   Group: iGEM23_IISER-Kolkata   (2023-10-11)


crRNA-XopN

This codes FnCas12a guide RNA against the Xanthomonas virulence associated gene XopN. XopN is an effector protein for Type-3 secretion(T3SS) system of Xanthomonas. Double strand break of XopN decreases the virulence of Xanthomonas.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None