Part:BBa_K4779001:Design
CMPG Pro:A yeast copper-induced reporter pathway with prm1 pro promoter
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Design Notes
When designing the sequence in detail, one of the key considerations is how to convert copper ion signals into information molecule signals more efficiently and sensitively. In this round of DBTL, we selected a more sensitive pprm1 Pro promoter to replace pprm1, thereby enhancing the expression intensity of GFP under the information molecule promoter. pprm1 Pro (BBa_K3384313) is a more sensitive information molecule-responsive promoter, containing three ×PREs in the same direction as the promoter, providing better sensitivity to information molecules.
Source
CMPG pro is a new composite part (BBa_K4779001). We obtained the gene sequences of the promoter of Cup1 (BBa_K945002), MFα2 (BBa_K110005), the promoter of prm1 pro (BBa_K3384313), green fluorescent protein (GFP) (BBa_K3112009), and the terminator CYC1 (BBa_K4278703) from IGEM, and sent them to GENEWIZ (Suzhou genewiz Biotechnology Co., Ltd.) for synthesis.