Composite

Part:BBa_K4774013:Design

Designed by: Wong Chak Sum   Group: iGEM23_HKPOLYU   (2023-09-22)


CBD-SsrA+Npu-peptide-Npu 2-CBD2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 684
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 684
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 684
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 684
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The composite part combines A linear EGFR-binding peptide (BBa_K4774000), Npu intein (BBa_K4774005 and BBa_K4774007), SsrA tag (BBa_K4774006) CBD (BBa_K4774002) and CBD2 (BBa_J70627)


Source

Genebank

References