Composite
Part:BBa_K4774012
Designed by: Wong Chak Sum Group: iGEM23_HKPOLYU (2023-09-22)
CBD-SsrA+Npu-eGFP-Npu 2-CBD2
Enhanced Green Fluorescent Protein (eGFP, BBa_K4401002) as a positive control to confirm Npu intein (BBa_K4774005 and BBa_K4774007) system is working for expressing a short EGFR-binding peptide in a plasmid with CBD (Chitin Binding Domain, BBa_K4774002 and BBa_J70627) for purification.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1363
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1363
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1363
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1363
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |