Composite

Part:BBa_K4774012

Designed by: Wong Chak Sum   Group: iGEM23_HKPOLYU   (2023-09-22)


CBD-SsrA+Npu-eGFP-Npu 2-CBD2

Enhanced Green Fluorescent Protein (eGFP, BBa_K4401002) as a positive control to confirm Npu intein (BBa_K4774005 and BBa_K4774007) system is working for expressing a short EGFR-binding peptide in a plasmid with CBD (Chitin Binding Domain, BBa_K4774002 and BBa_J70627) for purification.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1363
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1363
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1363
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1363
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None