Part:BBa_K4774010:Design

CBD-Ssp-peptide-Mxe-CBD2

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 1247
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 588
  Illegal SpeI site found at 1247
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 1247
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 1247
  Illegal NgoMIV site found at 751
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 831
  

Design Notes

The composite part combines A linear EGFR-binding peptide (BBa_K4774000), Ssp DnaB intein (BBa_K4774003), Mxe GyrA intein (BBa_K4774004), CBD (BBa_K4774002) and CBD2 (BBa_J70627)

Source

Genebank