Composite

Part:BBa_K4774010

Designed by: Wong Chak Sum   Group: iGEM23_HKPOLYU   (2023-09-22)


CBD-Ssp-peptide-Mxe-CBD2

One-step circularization of EGFR-binding peptide (BBa_K4774000) upon Ssp DnaB (BBa_K4774003) and Mxe GyrA intein (BBa_K4774004) splicing with CBD (Chitin Binding Domain, BBa_K4774002 and BBa_J70627) for purification.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1247
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 588
    Illegal SpeI site found at 1247
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1247
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1247
    Illegal NgoMIV site found at 751
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 831


[edit]
Categories
Parameters
None