Composite

Part:BBa_K4773012:Design

Designed by: Yan Pan   Group: iGEM23_UESTC-China   (2023-10-01)


Lac Promoter+RBS+SQR+RBS+SDO+RBS+AprBA+RBS+SAT+Term


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4152
    Illegal SpeI site found at 2897
    Illegal PstI site found at 3698
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5122
    Illegal SpeI site found at 2897
    Illegal PstI site found at 3698
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3097
    Illegal BamHI site found at 4357
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4152
    Illegal SpeI site found at 2897
    Illegal PstI site found at 3698
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4152
    Illegal SpeI site found at 2897
    Illegal PstI site found at 3698
    Illegal NgoMIV site found at 1338
    Illegal NgoMIV site found at 1762
    Illegal NgoMIV site found at 2823
    Illegal NgoMIV site found at 4831
    Illegal NgoMIV site found at 5002
    Illegal NgoMIV site found at 5685
    Illegal AgeI site found at 2257
    Illegal AgeI site found at 2844
    Illegal AgeI site found at 3509
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

RBS (BBa_B0034) was chosen because it has a high translation initiation rate and is compatible with most bacterial hosts.Term (BBa_B0015) was chosen because it is a double terminator, which can effectively terminate transcription and protect mRNA from degradation.


Source

SQR: From the genomic sequence of Acidithiobacillus thiooxidans. SDO: From the genomic sequence of Aquifex aeolicus. AprBA: From the genomic sequence of Desulfovibrio vulgaris. SAT: From the genomic sequence of Escherichia coli.

References