Part:BBa_K4773000:Design
RBS-NicA
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 123
Illegal BglII site found at 364 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The expression of NicA may be regulated by an inducible promoter, such as BBa_I13453 (pLacIq), to control the timing and level of nicotine degradation.
The expression of NicA may affect the growth and viability of the bacterial cells, due to the production of pseudooxynicotine and other toxic intermediates. Therefore, it may be necessary to optimize the culture conditions and/or provide detoxification mechanisms for the bacterial cells.
Source
Source: This part is derived from the genomic sequence of Pseudomonas putida S16, a nicotine-degrading bacterium isolated from tobacco waste. And was cloned into the pUSP vector with the BBa_B0034 RBS.
References
Tang H, Wang L, Wang W, Yu H, Zhang K, Yao Y, Xu P. Systematic unraveling of the unsolved pathway of nicotine degradation in Pseudomonas. PLoS Genet. 2013 Oct;9(10):e1003923. doi: 10.1371/journal.pgen.1003923. Epub 2013 Oct 31. PMID: 24204323; PMCID: PMC38143101
Wang L, Tang H, Wang S, Liu R, Liu M, Zhang Y, Chen T, He X, Li S, Xu P. Novel nicotine oxidoreductase-encoding gene involved in nicotine degradation by Pseudomonas putida strain S16. Appl Environ Microbiol. 2012 Jan;78(2):364-72. doi: 10.1128/AEM.06262-11. Epub 2011 Nov 4. PMID: 22057864; PMCID: PMC32556472