Coding
Part:BBa_K4772001:Design
Designed by: 清雯 李 Group: iGEM23_Tsinghua-TFL (2023-10-08)
ChlF: A photo-oxidoreductase that uses light to oxidize chlorophyll a to produce chlorophyll f.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 574
Illegal NgoMIV site found at 913 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part's sequence do not contain illegal restriction sites (or other specified sequences) as defined by the assembly standard. And it is compatible to iGEM Type II S Assembly Method.
Source
The sequence of ChlF is from Chlorogloeopsis fritschii PCC 9212 .The specific website address is as follows: https://www.ncbi.nlm.nih.gov/protein/1099384185. After we get the sequence, it underwent codon optimization according to Chlamydomonas reinhardtii and was synthesized by company.