Part:BBa_K4770032:Design

NR_NiR_Level_M

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 3882
  Illegal PstI site found at 5146
  Illegal PstI site found at 12173
  Illegal PstI site found at 13699
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 5726
  Illegal NheI site found at 7969
  Illegal PstI site found at 3882
  Illegal PstI site found at 5146
  Illegal PstI site found at 12173
  Illegal PstI site found at 13699
  Illegal NotI site found at 13435
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 6281
  Illegal BglII site found at 9699
  Illegal BglII site found at 10165
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 3882
  Illegal PstI site found at 5146
  Illegal PstI site found at 12173
  Illegal PstI site found at 13699
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 3882
  Illegal PstI site found at 5146
  Illegal PstI site found at 12173
  Illegal PstI site found at 13699
  Illegal NgoMIV site found at 1265
  Illegal NgoMIV site found at 1447
  Illegal NgoMIV site found at 3771
  Illegal NgoMIV site found at 4365
  Illegal NgoMIV site found at 4980
  Illegal NgoMIV site found at 6097
  Illegal NgoMIV site found at 8444
  Illegal AgeI site found at 3543
  Illegal AgeI site found at 5856
  Illegal AgeI site found at 9488
  
• 1000
  COMPATIBLE WITH RFC[1000]

Source of this part

Position 3: Hygro R cassette BBa_K4770016

Position 4: pCM1 NR: BBa_K4770018

Position 5: pCM1 NiR BBa_K4770019

Position 6: mCherry cassette BBa_K4770017

Position 7: mVenus cassette BBa_K4770025

Design considerations

AlgaGenix’s Level Ms are designed to have the same structure and not having to build different pieces with different positions for each transcriptional unit. Each piece has it’s own design considerations that can be looked up on the individual part’s pages.