Coding

Part:BBa_K4770030:Design

Designed by: Andrea Camí Bonet   Group: iGEM23_Barcelona-UB   (2023-10-12)

IPT_Isopentenyltransferase_Level_M


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3323
    Illegal PstI site found at 4455
    Illegal PstI site found at 4876
    Illegal PstI site found at 4900
    Illegal PstI site found at 7575
    Illegal PstI site found at 9101
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3323
    Illegal PstI site found at 4455
    Illegal PstI site found at 4876
    Illegal PstI site found at 4900
    Illegal PstI site found at 7575
    Illegal PstI site found at 9101
    Illegal NotI site found at 3203
    Illegal NotI site found at 4836
    Illegal NotI site found at 8837
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5567
    Illegal BamHI site found at 4195
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3323
    Illegal PstI site found at 4455
    Illegal PstI site found at 4876
    Illegal PstI site found at 4900
    Illegal PstI site found at 7575
    Illegal PstI site found at 9101
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3323
    Illegal PstI site found at 4455
    Illegal PstI site found at 4876
    Illegal PstI site found at 4900
    Illegal PstI site found at 7575
    Illegal PstI site found at 9101
    Illegal NgoMIV site found at 1265
    Illegal NgoMIV site found at 1447
    Illegal NgoMIV site found at 3253
    Illegal NgoMIV site found at 4267
    Illegal NgoMIV site found at 4533
    Illegal NgoMIV site found at 4937
    Illegal NgoMIV site found at 4949
  • 1000
    COMPATIBLE WITH RFC[1000]

Source of this part

Position 4: Hygro R cassette BBa_K4770016

Position 5: pCM1 IPT BBa_K4770022

Position 6: mCherry cassette BBa_K4770017

Position 7: mVenus cassette BBa_K4770025

Design considerations

AlgaGenix’s Level Ms are designed to have the same structure and not having to build different pieces with different positions for each transcriptional unit. Each piece has it’s own design considerations that can be looked up on the individual part’s pages.