Coding

Part:BBa_K4770029:Design

Designed by: Andrea Camí Bonet   Group: iGEM23_Barcelona-UB   (2023-10-12)

Cp_GS_Chloroplast_Glutamate_Synthetase_Level_M


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4830
    Illegal PstI site found at 7168
    Illegal PstI site found at 8686
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4830
    Illegal PstI site found at 7168
    Illegal PstI site found at 8686
    Illegal NotI site found at 8422
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4830
    Illegal BglII site found at 5168
    Illegal BamHI site found at 4474
    Illegal XhoI site found at 4686
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4830
    Illegal PstI site found at 7168
    Illegal PstI site found at 8686
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4830
    Illegal PstI site found at 7168
    Illegal PstI site found at 8686
    Illegal NgoMIV site found at 1265
    Illegal NgoMIV site found at 1447
    Illegal NgoMIV site found at 4032
    Illegal NgoMIV site found at 4984
    Illegal NgoMIV site found at 9163
  • 1000
    COMPATIBLE WITH RFC[1000]

Source of this part

Position 4: Hygro R cassette BBa_K4770016

Position 5: pCM1 Cp-GS BBa_K4770021

Position 6: mCherry cassette BBa_K4770017

Position 7: mVenus cassette BBa_K4770025

Design considerations

AlgaGenix’s Level Ms are designed to have the same structure and not having to build different pieces with different positions for each transcriptional unit. Each piece has it’s own design considerations that can be looked up on the individual part’s pages.