Coding

Part:BBa_K4770028:Design

Designed by: Andrea Camí Bonet   Group: iGEM23_Barcelona-UB   (2023-10-12)

GS_Glutamate_Synthetase_Level_M


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4725
    Illegal PstI site found at 7071
    Illegal PstI site found at 8597
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4725
    Illegal PstI site found at 7071
    Illegal PstI site found at 8597
    Illegal NotI site found at 8333
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4725
    Illegal BglII site found at 5063
    Illegal BamHI site found at 4369
    Illegal XhoI site found at 4581
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4725
    Illegal PstI site found at 7071
    Illegal PstI site found at 8597
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4725
    Illegal PstI site found at 7071
    Illegal PstI site found at 8597
    Illegal NgoMIV site found at 1265
    Illegal NgoMIV site found at 1447
    Illegal NgoMIV site found at 3927
    Illegal NgoMIV site found at 4879
    Illegal NgoMIV site found at 9074
  • 1000
    COMPATIBLE WITH RFC[1000]

Source of this part

Position 4: Hygro R cassette BBa_K4770016

Position 5: pCM1 GS BBa_K4770020

Position 6: mCherry cassette BBa_K4770017

Position 7: mVenus cassette BBa_K4770025

Design considerations

AlgaGenix’s Level Ms are designed to have the same structure and not having to build different pieces with different positions for each transcriptional unit. Each piece has it’s own design considerations that can be looked up on the individual part’s pages.